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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
06/10/2022 |
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Actualizado : |
06/10/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HANNAH BULLOCK, Synergy America Inc., Atlanta, GA 30329, USA; MARINA MAURENTE BERON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; NÉLIDA RODRÍGUEZ-OSORIO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; LUCIANA SILVA-FLANNERY, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS CABRERA, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay; RODRIGO PUENTES, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; JANA M. RITTER, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay. |
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Título : |
Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 |
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ISSN : |
1999-4915 |
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DOI : |
10.3390/v14092042 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com --
Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock |
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Contenido : |
ABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. MenosABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus mor... Presentar Todo |
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Palabras claves : |
Abortion; Cattle; Emerging diseases; Epsilonpolyomavirus bovis; Nephropathy; Next generation sequencing; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Polyomavirus; Reproductive diseases; Viral diseases. |
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Asunto categoría : |
L20 Ecología animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16810/1/viruses-14-02042-v2.pdf
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Marc : |
LEADER 04533naa a2200445 a 4500
001 1063641
005 2022-10-06
008 2022 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v14092042$2DOI
100 1 $aGIANNITTI, F.
245 $aBovine Polyomavirus-1 (Epsilonpolyomavirus bovis)$bAn emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022. Academic Editors: Fernando Bauermann and Mayara Maggioli. Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com -- Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock
520 $aABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors.
653 $aAbortion
653 $aCattle
653 $aEmerging diseases
653 $aEpsilonpolyomavirus bovis
653 $aNephropathy
653 $aNext generation sequencing
653 $aPathology
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPolyomavirus
653 $aReproductive diseases
653 $aViral diseases
700 1 $aSILVEIRA, C.S.
700 1 $aBULLOCK, H.
700 1 $aBERON, M.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRODRÍGUEZ-OSORIO, N.
700 1 $aSILVA-FLANNERY, L.
700 1 $aPERDOMO, T.
700 1 $aCABRERA, A.
700 1 $aPUENTES, R.
700 1 $aCOLINA, R.
700 1 $aRITTER, J.M.
700 1 $aCASTELLS, M.
773 $tViruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
29/07/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTELLS, M.; CAFFARENA, D.; CASAUX, M.L.; SCHILD, C.; MIÑO, S.; CASTELLS, F.; CASTELLS, D.; VICTORIA, M.; RIET-CORREA, F.; GIANNITTI, F.; PAREÑO, V.; COLINA, R. |
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Afiliación : |
MATÍAS CASTELLS BAUER, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay./INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Facultad de Veterinaria, Universidad de la República, Alberto Lasplaces 1620, Montevideo 11600, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SAMUEL MIÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires 1686, Argentina.; FELIPE CASTELLS, Doctor en Veterinaria en Ejercicio Libre, Asociado al Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.; DANIEL CASTELLS, Centro de Investigación y Experimentación Dr. Alejandro Gallinal, Secretariado Uruguayo de la Lana, Ruta 7 km 140, Cerro Colorado, Florida 94000, Uruguay.; MATIAS VICTORIA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIVIANA PAREÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires 1686, Argentina; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto 50000, Uruguay. |
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Título : |
Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Pathogens (Basel, Switzerland), July 2020, Volume 9, Issue 7, Article number 570, Pages 1-17. OPEN ACCESS. DOI: https://doi.org/10.3390/pathogens9070570 |
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DOI : |
10.3390/pathogens9070570 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 7 April 2020 / Revised: 20 June 2020 / Accepted: 30 June 2020 / Published: 14 July 2020.
This research was funded by Instituto Nacional de Investigación Agropecuaria (INIA), grant number PL_015 N-15156 and N-23398, and by the Universidad de la República Program Polo de Desarrollo Universitario. The APC was funded by Universidad de la República. |
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Contenido : |
Abstract:
Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans. MenosAbstract:
Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested intersp... Presentar Todo |
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Palabras claves : |
BOVINE; DIARRHEA; GENOTYPES; INTERSPECIES TRANSMISSION; PLATAFORMA SALUD ANIMAL; ROTAVIRUS. |
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Thesagro : |
GANADO BOVINO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16692/1/pathogens-09-00570.pdf
https://www.mdpi.com/2076-0817/9/7/570/pdf
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Marc : |
LEADER 03089naa a2200361 a 4500
001 1061250
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3390/pathogens9070570$2DOI
100 1 $aCASTELLS, M.
245 $aPhylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 7 April 2020 / Revised: 20 June 2020 / Accepted: 30 June 2020 / Published: 14 July 2020. This research was funded by Instituto Nacional de Investigación Agropecuaria (INIA), grant number PL_015 N-15156 and N-23398, and by the Universidad de la República Program Polo de Desarrollo Universitario. The APC was funded by Universidad de la República.
520 $aAbstract: Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.
650 $aGANADO BOVINO
653 $aBOVINE
653 $aDIARRHEA
653 $aGENOTYPES
653 $aINTERSPECIES TRANSMISSION
653 $aPLATAFORMA SALUD ANIMAL
653 $aROTAVIRUS
700 1 $aCAFFARENA, D.
700 1 $aCASAUX, M.L.
700 1 $aSCHILD, C.
700 1 $aMIÑO, S.
700 1 $aCASTELLS, F.
700 1 $aCASTELLS, D.
700 1 $aVICTORIA, M.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
700 1 $aPAREÑO, V.
700 1 $aCOLINA, R.
773 $tPathogens (Basel, Switzerland), July 2020, Volume 9, Issue 7, Article number 570, Pages 1-17. OPEN ACCESS. DOI: https://doi.org/10.3390/pathogens9070570
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